Test Code ACTHM Adrenocorticotropic Hormone, Plasma
Additional Codes
| Mayo | ACTH |
| Epic ID | LAB511 |
Specimen Type
Plasma EDTANecessary Information
Separate specimens should be submitted when multiple tests are ordered.
Specimen Required
Patient Preparation: For 12 hours before specimen collection, patient should not take multivitamins or dietary supplements (eg, hair, skin, and nail supplements) containing biotin (vitamin B7).
Supplies: Sarstedt 5 mL Aliquot Tube (T914)
Collection Container/Tube: Ice-cooled, lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Morning (7 a.m.-10 a.m.) specimen is desirable.
2. Collect with a pre-chilled lavender top (EDTA) tube and transport to the laboratory on ice.
3. Within 2 hours of collection centrifuge at refrigerated temperature and immediately separate plasma from cells.
4. Immediately freeze plasma.
Specimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma EDTA | Frozen (preferred) | 28 days |
| Refrigerated | 3 hours | |
| Ambient | 2 hours |
Transport frozen.
Reject Due To
| Gross hemolysis | Reject |
| Gross lipemia | OK |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Electrochemiluminescence Immunoassay
Useful For
Determining the cause of hypercortisolism and hypocortisolism
Day(s) Performed
Monday through Saturday
Report Available
1 to 3 daysReference Values
7.2-63 pg/mL (a.m. draws)
Reference ranges are based on samples drawn between 7 a.m.-10 a.m.
No established reference values for p.m. draws
Pediatric reference values are the same as adults, as confirmed by peer reviewed literature.
Petersen KE. ACTH in normal children and children with pituitary and adrenal diseases. I. Measurement in plasma by radioimmunoassay-basal values. Acta Paediatr Scand. 1981;70(3):341-345
For International System of Units (SI) conversion for Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html.
CPT Code Information
82024