Antineutrophil Cytoplasmic Antibodies Vasculitis Panel, Serum

Additional Codes

Mayo	VASC
Epic ID	LAB3503

Specimen Type

Serum

Ordering Guidance

For monitoring disease activity, order either PR3 / Proteinase 3 Antibodies, IgG, Serum or MPO / Myeloperoxidase Antibodies, IgG, Serum.

Specimen Required

Supplies: Sarstedt Aliquot Tube 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type	Temperature	Time
Serum	Refrigerated (preferred)	21 days
	Frozen	21 days

UPH Waterloo Region Lab Test Catalog Note:

Transport refrigerated.

Reject Due To

Gross hemolysis	Reject
Gross lipemia	Reject
Gross icterus	OK

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Multiplex Flow Immunoassay

Profile Information

Test ID	Reporting Name	Available Separately	Always Performed
MPO	Myeloperoxidase Ab, S	Yes	Yes
PR3	Proteinase 3 Ab (PR3), S	Yes	Yes

Testing Algorithm

If either the myeloperoxidase antibody or proteinase 3 antibody result is greater than or equal to 0.4 U, then cytoplasmic neutrophilic antibody testing will be performed at an additional charge.

Reflex Tests

Test ID	Reporting Name	Available Separately	Always Performed
ANCA	Cytoplasmic Neutrophilic Ab, S	Yes	No

Useful For

Evaluating patients with clinical features of anti-neutrophil cytoplasmic antibody-associated vasculitis, specifically granulomatosis with polyangiitis, microscopic polyangiitis, and eosinophilic granulomatosis with polyangiitis

Day(s) Performed

Monday through Saturday

Report Available

3 to 4 days

Reference Values

MYELOPEROXIDASE ANTIBODIES, IgG

<0.4 U (negative)

0.4-0.9 U (equivocal)

≥1.0 U (positive)

Reference values apply to all ages.

PROTEINASE 3 ANTIBODIES, IgG