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HelpTest Code COCOUM Cortisol/Cortisone, Free, 24 Hour, Urine
Additional Codes
| Mayo | COCOU |
| Epic ID | LAB4519 |
Special Instructions
Specimen Type
UrineNecessary Information
24-Hour volume (in milliliters) is required.
Specimen Required
Supplies: Urine Tubes, 10-mL (T068)
Submission Container/Tube: Plastic, urine tube (T068)
Specimen Volume: 5 mL
Collection Instructions:
1. Add 10 g of boric acid as preservative at start of collection.
2. Collect urine for a full 24 hours (required) and record the total volume.
Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens for multiple collections.
Collect 5 mL of a 24 hour urine. Refrigerate during and after collection. No preservative needed.
Specimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated (preferred) | 14 days |
| Frozen | 28 days | |
| Ambient | 72 hours |
Transport refrigerated.
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Useful For
Screening test for Cushing syndrome (hypercortisolism)
Assisting in diagnosing acquired or inherited abnormalities of 11-beta-hydroxy steroid dehydrogenase (cortisol to cortisone ratio)
Diagnosis of pseudo-hyperaldosteronism due to excessive licorice consumption
This test has limited usefulness in the evaluation of adrenal insufficiency.
Day(s) Performed
Monday through Friday
Report Available
2 to 7 daysReference Values
CORTISOL
0-2 years: not established
3-8 years: 1.4-20 mcg/24 h
9-12 years: 2.6-37 mcg/24 h
13-17 years: 4.0-56 mcg/24 h
≥18 years: 3.5-45 mcg/24 h
CORTISONE
0-2 years: not established
3-8 years: 5.5-41 mcg/24 h
9-12 years: 9.9-73 mcg/24 h
13-17 years: 15-108 mcg/24 h
≥18 years: 17-129 mcg/24 h
Use the factors below to convert each analyte from mcg/24 h to nmol/24 h:
Conversion factors
Cortisol: mcg/24 hours x 2.76=nmol/24 h (molecular weight=362.5)
Cortisone: mcg/24 hours x 2.78=nmol/24 h (molecular weight=360)
For International System of Units (SI) conversion for Reference Values, see www.mayocliniclabs.com/order-tests/si-unit-conversion.html
CPT Code Information
82530-Cortisol; free
82542