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HelpTest Code PHI11M Prostate Health Index Reflex, Serum
Additional Codes
| Mayo | PHI11 |
| Epic ID | LAB3751 |
Specimen Type
Serum RedSpecimen Required
Patient Preparation:
1. Specimens for testing should be collected prior to prostate manipulations such as digital rectal examination, prostatic massage, transrectal ultrasound, and prostatic biopsy.
2. A 6-week waiting period between needle biopsy and specimen collection is recommended.
3. Specimens should not be collected from patients receiving therapy with high biotin (vitamin B7) doses (ie, >5 mg/day) until at least 8 hours following the last biotin administration.
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube: Red top (serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Within 3 hours of collection, centrifuge, aliquot serum into a plastic vial, and refrigerate. Freeze sample within 24 hours of collection and send frozen.
Specimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Frozen (preferred) | 150 days |
| Ambient | 24 hours | |
| Refrigerated | 24 hours |
Transport frozen.
Reject Due To
| Gross hemolysis | Reject |
| Gross icterus | Reject |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Immunoenzymatic Assay
Testing Algorithm
This test begins with the analysis of prostate-specific antigen (PSA). If the PSA concentration is between 2.0 and 10.0 ng/mL, then the reflex test will be performed at an additional charge.
If the initial PSA concentration is between 2.0 and 10.0 ng/mL, then free PSA and [-2]pro-PSA isoform results will be reported.
If the initial PSA concentration is between 4.0 and 10.0 ng/mL, then the percent free PSA result and prostate health index (phi) will be calculated and reported.
If the initial PSA concentration is between 2.0 and 4.0 ng/mL, then the percent free PSA result and prostate health index (phi) will not be calculated or reported.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| PHI13 | Prostate Health Index (phi) Reflex | No | No |
Useful For
As an aid in distinguishing prostate cancer from benign prostatic conditions in men aged 50 years and older with total PSA between 4.0 and 10.0 ng/mL and digital rectal examination findings that are not suspicious for cancer
Day(s) Performed
Monday through Saturday
Report Available
1 to 3 daysReference Values
Females: Not applicable
PROSTATE-SPECIFIC ANTIGEN (PSA) MALES:
|
Age |
Reference range |
|
<40 years |
≤2.0 ng/mL |
|
40-49 years |
≤2.5 ng/mL |
|
50-59 years |
≤3.5 ng/mL |
|
60-69 years |
≤4.5 ng/mL |
|
70-79 years |
≤6.5 ng/mL |
|
≥80 years |
≤7.2 ng/mL |
PERCENT FREE PSA MALES:
When PSA is in the range of 4.0-10.0 ng/mL
|
% Free PSA |
Probability of cancer |
|
≤10% |
56% |
|
11-15% |
28% |
|
16-20% |
20% |
|
21-25% |
16% |
|
>25% |
8% |
PROSTATE HEALTH INDEX (phi) MALES:
When PSA is in the range of 4.0-10.0 ng/mL
|
phi range |
Probability of cancer |
95% Confidence interval |
|
0-26.9 |
9.8% |
5.2-15.4% |
|
27.0-35.9 |
16.8% |
11.3-22.2% |
|
36.0-54.9 |
33.3% |
26.8-39.9% |
|
≥55.0 |
50.1% |
39.8-61.0% |
CPT Code Information
84153 - Total PSA
84154 - Free PSA (if appropriate)
86316 - [-2]ProPSA (if appropriate)