Test Code PPFEM Protoporphyrins, Fractionation, Whole Blood
Additional Codes
| Mayo | PPFE |
| Epic ID | LAB5282 |
Special Instructions
Specimen Type
Whole bloodOrdering Guidance
This test is for assessment for protoporphyria. The preferred test for lead toxicity in children is blood lead. For more information see PBDV / Lead, Venous with Demographics, Blood or PBDC / Lead, Capillary, with Demographics, Blood.
The preferred test for the assessment of occupational exposure to lead is PBPFD / Lead Profile Occupational Exposure, Blood.
The preferred screening test for suspicion of a hepatic porphyria is urine porphyrins. For more information see PQNRU / Porphyrins, Quantitative, Random, Urine. This test should not be ordered with PEE / Porphyrins Evaluation, Whole Blood
Necessary Information
Include a list of medications the patient is currently taking.
Specimen Required
All porphyrin tests on whole blood can be performed on 1 tube.
Patient Preparation: Patient must not consume any alcohol for 24 hours before specimen collection.
Container/Tube:
Preferred: Green top (sodium heparin)
Acceptable: Lavender top (EDTA), dark blue top (metal free heparin), royal blue top (metal free EDTA), green top (lithium heparin)
Specimen Volume: 4 mL
Collection Instructions: Refrigerate specimen as soon as possible.
Specimen Minimum Volume
3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole blood | Refrigerated | 7 days |
Transport whole blood refrigerated.
Reject Due To
| Gross hemolysis | Reject |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
High-Performance Liquid Chromatography (HPLC) with Fluorescence Detection
Testing Algorithm
This test should not be ordered in conjunction with PEE / Porphyrins Evaluation, Whole Blood.
The following information is available:
-Porphyria (Acute) Testing Algorithm
Useful For
Evaluating patients with possible diagnoses of erythropoietic protoporphyria or X-linked dominant protoporphyria
Establishing a biochemical diagnosis of erythropoietic protoporphyria and X-linked dominant protoporphyria
Day(s) Performed
Monday through Friday
Report Available
3 to 5 daysReference Values
FREE PROTOPORPHYRIN
<20 mcg/dL
ZINC-COMPLEXED PROTOPORPHYRIN
<60 mcg/dL
CPT Code Information
82542