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HelpTest Code SEMBM Semen Analysis with Strict Morphology, Semen
Additional Codes
| Mayo | SEMB |
| Epic ID | LAB4501 |
Specimen Type
SemenOrdering Guidance
This test should not be used to check patient's sterility following a vasectomy. For such cases, order POSV / Post Vasectomy Check, Semen.
Semen analysis specimens submitted to Mayo Clinic Laboratories are not acceptable for fructose testing due to the use of dilution media. For specimen requirements for fructose testing in azoospermia patients, see FROS2 / Fructose, Qualitative, Semen.
Submit separate specimen to rule-out ejaculatory duct blockage. Positive result indicates no blockage.
Shipping Instructions
Specimen must arrive within 24 hours of collection. Send specimen Monday through Thursday only and not the day before a holiday. If holiday falls on a Saturday, holiday will be observed on the preceding Friday. Sunday holidays are observed on the following Monday. Specimen should be collected and packaged as close to shipping time as possible. Laboratory does not perform testing on weekends.
Necessary Information
Include the following information:
-Semen volume (required)
-Viscosity
-pH
-Appearance (color)
-Number of days of sexual abstinence
Specimen Required
Patient Preparation: Patient should have 2 to 7 days of sexual abstinence at the time of semen collection for accurate results.
Supplies: Semen Analysis Kit - Dilution Media (T178)
Specimen Volume: Total ejaculate
Collection Instructions:
See Semen Collection Specimen Preparation and Packaging Instructions included with the kit
1. Prior to use, allow dilution medium to warm to room temperature for 45 to 60 minutes.
2. Allow semen to liquefy at room temperature for up to 30 minutes.
3. Use sterile volumetric pipet or tube for volume measurement.
4. Pour liquefied semen into 50-mL dilution medium container within 60 minutes of semen collection time, cap tightly, but do not overtighten, and gently mix.
Note: Proper temperature maintenance of specimen throughout processing and shipping is critical. All materials the specimen is exposed to should be at room temperature (20–28° C).
Collection of the semen specimen should follow a period of sexual abstinence of 2-7 days. 1.Specimen is collected by masturbation into a sterile container. (Sterile urine cup is acceptable). 2.Specimens are accepted by Allen Lab for processing and transport to Mayo on Monday-Thursdays. Specimens must arrive by noon. No holidays. 3. Deliver to the laboratory immediately (within 30 minutes of collection) keeping the specimen at approximately body temperature. (Wrap the container in a dry towel or washcloth). 4. Patient must complete the questionnaire on the collection of semen specimen procedure.
Specimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Semen | Ambient | 36 hours |
Transport at room temperature.
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
FER: Manual
MSCT: Kruger Criteria Strict Morphology
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| FER | Semen Analysis | Yes | Yes |
| MSTC | Strict Criteria Sperm Morphology | Yes | Yes |
Useful For
Determining male fertility status
Selecting the most cost-effective therapy for treating male-factor infertility
Quantifying the number of germinal and white blood cells per milliliter of semen
Day(s) Performed
Monday through Friday
Report Available
1 to 4 daysReference Values
SEMEN ANALYSIS
Appearance: normal
Volume: ≥1.5 mL
pH: ≥7.2
Motile/mL: ≥6.0 x 10(6)
Sperm/mL: ≥15.0 x 10(6)
Motility: ≥40%
Grade: ≥2.5
Note: Multiple laboratory studies have indicated that semen parameters for motility and grade on average retain 80% of original parameters when our shipping method is used for transport. Using these averages, samples with 32% to 39% motility and grade of 2 may be in the normal range if testing was performed shortly after collection. Therefore, these borderline patients may need to collect another sample at a local fertility center to verify fertility status.
Motile/ejaculate: ≥9.0 x 10(6)
Viscosity: ≥3.0
Agglutination: ≥3.0
Supravital: ≥58% live
Fructose: positive
Note: Fructose testing cannot be performed on semen analysis specimens shipped through Mayo Clinic Laboratories. If patient is azoospermic, refer to FROS2 / Fructose, Qualitative, Semen. Submit separate specimen to rule-out ejaculatory duct blockage. Positive result indicates no blockage.
STRICT MORPHOLOGY
Normal forms: ≥4.0% normal oval sperm heads
Germ cells: <4 x 10(6) (normal)
≥4 x 10(6)/mL (elevated germinal cells in semen are of unknown clinical significance)
White blood cell count:
<1 x 10(6) (normal)
≥1 x 10(6)/mL (elevated white blood cells in semen are of questionable clinical significance)
CPT Code Information
89310-Semen Analysis
89398-Strict Criteria Sperm Morphology
If both components performed,
89322-Semen Analysis with Strict Morphology