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Test Code SRAUM Serotonin Release Assay, Unfractionated Heparin, Mass Spectrometry, Serum

Additional Codes

Mayo SRAU
Epic ID LAB3977

 

Specimen Type

Serum Red


Shipping Instructions


Specimen should be frozen when shipped or stored for longer than 48 hours.



Specimen Required


Patient Preparation:

1. Fasting: 8 hours, preferred but not required; Collect during an episode of suspected heparin-induced thrombocytopenia

2. Patient should not be on ticagrelor (Brilinta) as this may interfere with the assay, yielding a false-negative result.

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Red top (serum gel/SST are not acceptable)

Submission Container/Tube: 13-mm x 75-mm, 5-mL, plastic vial (polypropylene preferred)

Specimen Volume: 1 mL Serum

Collection Instructions:

1. After collection, allow the specimen to sit for a minimum of 1 hour at ambient temperature to ensure it clots completely.

2. Within 48 hours of collection, centrifuge and aliquot serum into a 13-mm x 75-mm, 5-mL, polypropylene vial


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Red Frozen (preferred)
  Refrigerated  7 days
UPH Waterloo Region Lab Test Catalog Note:

Transport frozen.

Reject Due To

Gross lipemia Reject

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Useful For

Detecting heparin-dependent platelet activating antibodies implicated in the pathogenesis of heparin-induced thrombocytopenia

Day(s) Performed

Monday through Friday

Report Available

1 to 4 days

Reference Values

Low Heparin Serotonin Release: <20%

High Heparin Serotonin Release: <20%

Serotonin Release Assay Result: Negative

CPT Code Information

82542