Test Code SRAUM Serotonin Release Assay, Unfractionated Heparin, Mass Spectrometry, Serum
Additional Codes
| Mayo | SRAU |
| Epic ID | LAB3977 |
Specimen Type
Serum RedShipping Instructions
Specimen should be frozen when shipped or stored for longer than 48 hours.
Specimen Required
Patient Preparation:
1. Fasting: 8 hours, preferred but not required; Collect during an episode of suspected heparin-induced thrombocytopenia
2. Patient should not be on ticagrelor (Brilinta) as this may interfere with the assay, yielding a false-negative result.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (serum gel/SST are not acceptable)
Submission Container/Tube: 13-mm x 75-mm, 5-mL, plastic vial (polypropylene preferred)
Specimen Volume: 1 mL Serum
Collection Instructions:
1. After collection, allow the specimen to sit for a minimum of 1 hour at ambient temperature to ensure it clots completely.
2. Within 48 hours of collection, centrifuge and aliquot serum into a 13-mm x 75-mm, 5-mL, polypropylene vial
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Frozen (preferred) | |
| Refrigerated | 7 days | |
Transport frozen.
Reject Due To
| Gross lipemia | Reject |
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Useful For
Detecting heparin-dependent platelet activating antibodies implicated in the pathogenesis of heparin-induced thrombocytopenia
Day(s) Performed
Monday through Friday
Report Available
1 to 4 daysReference Values
Low Heparin Serotonin Release: <20%
High Heparin Serotonin Release: <20%
Serotonin Release Assay Result: Negative
CPT Code Information
82542